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. 2025 Nov 13;15:39780. doi: 10.1038/s41598-025-23545-3

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Protein-based phylogenetic analysis of phage Premi. A) A dendrogram representing the global genomic comparison of predicted proteins from phage Premi with other viral sequences by tBLASTx using VipTree. The colored rings surrounding the dendrogram represent current ICTV virus families (inner ring) and host groups (outer ring). The red star represents the position where phage Premi is located. B) An excerpt of the proteomic tree showing phage Premi and the 24 viruses that are most closely classified. C) At left, pairwise whole genome dot plots. At right, predicted proteins and identity detected by tBLASTx between Proteus phages Premi (OR798392) and PM 85 (NC_027379) or Escherichia phage T7 (NC_001604) in a syntenic alignment. Analysis performed using VipTree Proteomic Tree online server.