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Fig. 4. Effect of overexpression of RGA1ΔLIM on cell morphology and checkpoint function in bem2Δ cells. (A) The strains DLY1 (BEM2) and DLY 4015 (bem2Δ) containing either empty plasmid (YEplac195) or RGA1ΔLIM (pDLB2129) were grown to exponential phase in dextrose-containing medium at 30°C. Images of live cells were captured using DIC microscopy. (B) The indicated strains were assayed for checkpoint function as in Figure 2A.
