In the published paper, we have recently identified several errors. Specifically, the protein bands of P53 in Figure 2A and α-tubulin in Figure 9A were incorrectly transmitted. In addition, AI-based image analysis raised concerns regarding potential image duplication in these figures. Given the large number of pathological images included in the article, we also found layout oversights in Figures 7B, 10C and 10D. Furthermore, the flow cytometry results in Figure 11C and 11D were inadvertently misplaced. We hereby issue this erratum to provide the corrected figures and record the relevant updates. Importantly, these corrections do not alter the original conclusions of the research. The authors sincerely apologize for any confusion or concern these errors may have caused.The corrected versions of Figures 2, 7, 9, 10 and 11 are presented below.
Figure 2.
ASPH mediates resistance to doxorubicin (DOX)-induced DNA damage response. (A) ASPH downregulates key components of the error-free DNA damage repair machinery in response to DOX, including sensors and executors of double-strand break (DSB) and single-strand break (SSB) repair pathways (e.g., homologous recombination, HR). ASPH disrupts the integrity of the active/functional MRN complex, thereby promoting anti-apoptotic effects. (B-K) Quantification of dynamic changes in the expression profiles of the components shown in (A). (L) Biomarkers of DOX-induced DNA damage response in 4T1 cells. (M-P) Quantification of expression levels of the elements shown in (L). *P < 0.05; **P < 0.01; ***P < 0.001 versus corresponding control. Data are presented as mean ± SD from experiments performed in triplicate under each condition.
Figure 9.
Key elements involved in immunogenic cell death (ICD) in mice across different groups. (A, B) Dynamic changes in the expression profiles of the ER stress marker eIF2α, the “eat me” signal calreticulin (CALR), and HMGB1 on tumor cells, as well as HMGB1, a ligand of TLR4 on dendritic cells (DCs). (C) Expression and subcellular localization of ICD components in mice from different groups. (D-G) Quantification of results shown in (C). (H, I) Release of HMGB1 and IFNγ in mouse serum across different groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 versus corresponding control (Student’s t-test). Data are presented as mean ± SD from experiments performed in triplicate under each condition.
Figure 7.
Multi-organ metastasis in a murine model of breast cancer. (A) Gross morphology of primary and metastatic tumors in various organs from the control group. (B) Histopathological features corresponding to (A). (C) Lymphatic metastasis across different groups. *P < 0.05; **P < 0.01; ***P < 0.001 versus corresponding control (Student’s t-test). Data are presented as mean ± SD from experiments performed in triplicate under each condition.
Figure 10.
ASPH-based λ phage vaccine enhances antitumor immune responses. (A) Schematic representation of the in vitro cytotoxicity assay. (B) Compared with the control group, cytotoxicity of splenocytes in mice from the vaccine group increased by approximately 45-fold. (C, D) Expression profiles of markers for activated and matured dendritic cells (DCs) as well as tumor-infiltrating lymphocytes (TILs) in (C) primary mammary tumors and (D) pulmonary metastatic lesions from mice in different groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 versus corresponding control (Student’s t-test). Data are presented as mean ± SD from experiments performed in triplicate under each condition.
Figure 11.
ASPH-based λ phage vaccine stimulates adaptive immune responses. A. Expression profile of the dendritic cell (DC) maturation marker TLR4 in the spleen of mice from different groups. B. Infiltration of matured DCs and CD3+ T lymphocytes into pulmonary lesions of mice from the DOX + Vaccine group. C, D. Compared with the control group, the combinatorial treatment significantly enhanced activation of CD4+ and CD8+ T cells, contributing to the antitumor response in mice from the DOX + Vaccine group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 versus corresponding control (Student’s t-test). Data are presented as mean ± SD from experiments performed in triplicate under each condition.