Fig. 4. Complex formation with mixtures of disintegration substrates. (A) Assembly reactions containing the indicated substrate or an equimolar mixture of substrates were analyzed on a 5% native polyacrylamide gel in the absence of a divalent metal ion. S-53* and S-92 contain complementary target cores while S-53 and S-92 contain identical target cores. The position of the hybrid complex is indicated with an arrow. Only the portion of the gel containing higher order complexes is shown. (B) DNA was eluted from the indicated bands of the native gel in (A), purified and subjected to electrophoresis on an 8% denaturing polyacrylamide gel.