Fig. 5. DNA sequence dependencies for STT formation. S-53 and variants of this substrate were mixed with transposase in the absence of a divalent metal ion, and the ability of these substrates to form STT was determined as in Figure 2A. STT levels are presented as the percentage of total input substrate converted to STT. Asterisks indicate the approximate position of base pair substitutions. S-53 OE-S, OE-D and OE-T contain single (9TA to CG), double (8AT to CG, 9TA to CG) and triple (8AT to CG, 9TA to CG, 13CG to TA) mutations, respectively, in the outside end. S-53 TC-T contains a triple mutation (–2AT to TA, –3CG to GC, –8CG to AT) in the target core. Substrates are illustrated as in Figure 1D.