Table I. Oligonucleotides used in the assembly of STTs.
| Group | Oligo | Substrate | Sequence (5′-3′) |
|---|---|---|---|
| A | NT26 | CTGATGAATCCCCTAATGATTTTGGT | |
| TT31 | S-31 | ACCAAAATCATTAGGGGATTCATCAGCGCTG | |
| TT35 | S-35 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTG | |
| TT39 | S-39 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTA | |
| TT43 | S-43 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATT | |
| TT53 | S-53 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATTTTAATTTACA | |
| TTC53 | S-53* | ACCAAAATCATTAGGGGATTCATCAGCACTCAGCGTGTAAATTTTAATTTACA | |
| TMT53 | S-53 TC-T | ACCAAAATCATTAGGGGATTCATCAGCTGTGAGAGTGTAAATTTTAATTTACA | |
| TT56 | S-56 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATTTTAATTTACACAC | |
| TT59 | S-59 | ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATTTTAATTTACACACTCA | |
| |
TT62 |
S-62 |
ACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATTTTAATTTACACACTCAGCG |
| B | NT26 | S-53 | CTGATGAATCCCCTAATGATTTTGGT |
| TS26 | ACCAAAATCATTAGGGGATTCATCAG | ||
| T27 | CGCTGAGTGTGTAAATTTTAATTTACA | ||
| |
B19 |
|
CACTCAGCGCTGATGAATC |
| C | NTS26 | S-53 OE-S | CTGATGAACCCCCTAATGATTTTGGT |
| |
TTS53 |
|
ACCAAAATCATTAGGGGGTTCATCAGCGCTGAGTGTGTAAATTTTAATTTACA |
| D | NTD26 | S-53 OE-D | CTGATGACTCCCTTAATGATTTTGGT |
| |
TTD53 |
|
ACCAAAATCATTAAGGGAGTCATCAGCGCTGAGTGTGTAAATTTTAATTTACA |
| E | NTT26 | S-53 OE-T | CTGATGACCCCCTTAATGATTTTGGT |
| |
TTT53 |
|
ACCAAAATCATTAAGGGGGTCATCAGCGCTGAGTGTGTAAATTTTAATTTACA |
| F | NT65 | S-92 | CTGATGAATCCCCTAATGATTTTGGTAAAAATCTTAAGTTAAGGTGGATACACATCTTGTCATAT |
| TT92 | ATATGACAAGATGTGTATCCACCTTAACTTAATGATTTTTACCAAAATCATTAGGGGATTCATCAGCGCTGAGTGTGTAAATTTTAATTTACA |
Group designations define oligos that were mixed together to generate the indicated substrates. For group A, NT26 was mixed with only one of the other indicated oligos.