Table II. Oocyte development, maturation rates and embryonic lethality in lip-1(0) mutants and suppression by RAS/MAPK pathway mutations.
| Genotype | No. of oocytes in proximal arma (n) | % premature disappearance of the nucleolusb (n) | Maturations/hc (n) | % embryonic lethalityc (n) |
|---|---|---|---|---|
| Wild type | 7.9 ± 1.6 (46) | 0 (55) | 2.2 ± 0.2 (20) | 1.2 ± 1.3 (242) |
| lip-1(0) | 12.3 ± 4.1 (60) | 66 ± 12 (59) | 2.2 ± 0.3 (20) | 49.8 ± 4.5 (469) |
| lip-1(0) let-60(n2021) | 8.4 ± 2.5 (43) | 23 ± 14 (31) | 1.9 ± 0.5 (20) | 27.4 ± 4.7 (347) |
| lip-1(0); sem-5(n2019) | 7.9 ± 2.4 (29) | 34 ± 17 (29) | n.d. | 25.4 ± 6.4 (177) |
aThe average number of oocytes was scored by counting the number of cellularized oocytes in the proximal gonad arm of 2- to 3-day-old hermaphrodites.
bIn wild-type animals, the oocyte nucleolus disappears in either the second or the third oocyte prior to ovulation (McCarter et al., 1999; Page et al., 2001). The percentage premature nucleolar breakdown indicates the fraction of animals in which the disappearance of the nucleolus was observed before the third oocyte prior to ovulation. The disappearance of the nucleolus was observed in the fourth oocyte prior to ovulation in 29% of the lip-1(0) gonads, and in 37% before the fourth oocyte (n = 59).
cMaturation rates and embryonic lethality were scored as described in Materials and methods. Where appropriate, the 95% confidence intervals are indicated. In columns 2 and 3, (n) refers to the number of gonads examined, in column 4 to the number of animals and in column 5 to the number of embryos analysed.