Table IV. Inactivation of LIP-1 in G2/M arrested oocytes.
| Row | Genotype | Temperaturea | % Emob | nc |
|---|---|---|---|---|
| 1 | Wild type | 14°C | 0 | 51 |
| 2 | Wild type | 25°C | 6 ± 8 | 35 |
| 3 | lip-1(0) | 14°C | 53 ± 23 | 17 |
| 4 | lip-1(0) | 25°C | 100 | 25 |
| 5 | lip-1(zh32) | 14°C | 4 ± 6 | 48 |
| 6 | lip-1(zh32) | 25°C | 87 ± 9 | 55 |
| 7 | lip-1(zh32) | Up-shift to 25°C | 14 ± 8 | 70 |
aIn order to feminize the animals without using a temperature-sensitive fem mutation, hermaphrodites were grown at the indicated temperature until they had stopped producing fertilized eggs (5 days after reaching the L4 stage at 25°C and 7 days after L4 at 14°C). For the up-shift experiment shown in row 7, the animals were grown at 14°C for 7 days and then placed at 25°C for another 2 days.
bThe Emo phenotype was scored by examining adults for the presence of endomitotic oocytes in the proximal gonad arm by DAPI staining or using Nomarski optics (rows 3 and 4). Where appropriate, the 95% confidence intervals are indicated.
cn refers to the number of animals scored.