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. 2025 Nov 14;15:40047. doi: 10.1038/s41598-025-24530-6

Table 3.

Effects of N application on N accumulation amount different growth stage.

Treatment Wintering stage Jointing stage Booting stage Anthesis stage Maturity stage
2020–2021 N0 39.06 cd 63.28 e 138.29 e 160.96 e 191.41 f.
N90 41.94 bc 68.18 d 148.54 d 180.07 d 212.85 e
N180 43.45 b 75.43 c 154.08 c 188.16 c 223.53 d
N210 44.42 b 78.03 bc 165.76 b 192.74 c 228.54 c
N240 47.15 a 92.23 a 177.87 a 220.05 a 262.61 a
N270 44.59 b 78.98 b 147.34 d 205.86 b 241.44 b
N300 37.26 d 63.89 e 144.42 d 181.26 d 215.00 e
2021–2022 N0 30.21 d 53.28 d 118.32 f. 145.76 d 174.76 e
N90 35.54 c 58.74 c 125.45 e 160.61 bc 197.23 d
N180 38.57 bc 62.13 bc 134.38 cd 168.46 b 207.58 c
N210 43.52 a 68.50 a 145.66 a 185.44 a 229.61 a
N240 41.65 ab 62.52 b 141.27 b 182.45 a 226.28 ab
N270 39.67 b 58.59 c 137.54 c 179.60 a 223.09 b
N300 31.43 d 51.38 d 134.82 cd 158.68 c 200.25 d
 F-value
 Year (Y) * * * * *
 Nitrogen (N) ** ** ** ** **
 Y × N ns * ns ** ns

The means are not significantly different within a given season when followed by the same lowercase letter using LSD at p < 0.05. “*” and “**”, indicate significant difference between the two optimal N rates at 0.01 and 0.05 probability levels, respectively.