Fig. 1. Analysis of the truncated LPG accumulated in the L.donovani mutant OB1. (A) Structure of the LPG from L.donovani and OB1. (B) The [³H]mannose-labeled truncated LPG from OB1 was subjected to nitrous acid deamination and then applied to DEAE cellulose as described in Materials and methods. (C) The (–2) charged, [³H]mannose-labeled glycan from (B) (fractions 21–28) was hydrolyzed with mild acid into neutral and (–4) charged fragments, as depicted in the reaction scheme with the radioactive mannose residues indicated with asterisks, which were separated by anion exchange chromatography. The graph shows Dionex HPLC chromatography of the neutral fragment as described previously (Mahoney et al., 1999). The elution positions of standard monosaccharides are shown. (D) The (–4) charged fragment described in (C) was further treated with alkaline phosphatase and then analyzed by Dionex HPLC as described previously (Mahoney et al., 1999). Standards: a, Glc; b, Gal-Man; c, maltose; d, maltotriose; e, maltopentaose; f, maltohexaose; g, maltoheptaose.