Fig. 9. Binding of Leishmania proteins to an LPG3–GFP fusion. Lysates from OB1 and WT Leishmania cells transfected with either pXG-LPG3^wt-GFP or pXG-LPG3^OB1-GFP were immunoprecipitated with anti-GFP antibodies (Clontech) and protein A–Sepharose as described in Materials and methods. Samples were subjected to SDS–PAGE (10% gel) under reducing conditions and silver-stained to visualize protein bands. Three distinct proteins are visible and labeled 1, 2 and 3. Band 1 corresponds to the LPG3–GFP fusion protein and band 2 has a size similar to the predicted LPG3 protein as discussed in the text. The identity of the third band (75 kDa) is unknown.