Fig. 3. GDI1^– cells display cytokinesis and growth defects. (A) DAPI staining (top panels) and corresponding phase contrast (bottom panels) images of wild-type and mutant cells grown on glass coverslips. Scale bar, 50 µm. (B) Distribution of the number of nuclei in GDI1^– and complementation mutants grown on coverslips. In the GDI1^– mutant, cells with four or more nuclei account for ∼10% of the population. (C) Growth of GDI1 null and complementation mutants in shaking suspension. The growth and cytokinesis defects of GDI1^– were restored after re-expression of GDI1. Curves are representative of at least three independent determinations, each performed in duplicate.