Table 5.
Amino acid (aa) substitutions identified at positions Pro‐197 and Trp‐574 of the ALS gene in P. annua populations. Eight plants per population were analyzed. The number of plants in which specific mutation(s) were detected are given in parenthesis. Sequence alignment analysis (ALS1 and ALS2) was conducted only on ALS positions that showed a mutation in the pyrosequencing. Mutant aa substitutions are in bold
| ALS pyrosequencing results | ALS1 | ALS2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pro (CCG)‐197 | Trp (TGG)‐574 | Pro (CCG)‐197 | Trp (TGG)‐574 | Pro (CCG)‐197 | Trp (TGG)‐574 | |||||||
| Population | Codon | aa | Codon | aa | Codon | aa | Codon | aa | Codon | aa | Codon | aa |
| POAAN‐R1 | C/A‐CG (8) | Pro/Thr | TGG (8) | Trp | CCG (8) | Pro | ACG (8) | Thr | ||||
| POAAN‐R2 | C‐C/A‐G (8) | Pro/Gln | TGG (8) | Trp | CAG (8) | Gln | CCG (8) | Pro | ||||
| POAAN‐R3 | C‐C/A‐G (5), C/A‐CG (3) | Pro/Gln, Pro/Thr | TGG (8) | Trp | CCG (3), CAG (5) | Pro, Gln | CCG (5), ACG (3) | Pro, Thr | ||||
| POAAN‐R4 | C/A‐CG (7) | Pro/Thr | T‐G/T‐G (1) | Trp/Leu | CCG (8) | Pro | TGG (8) | Trp | ACG (7) | Thr | TGG (7), TTG (1) | Trp, Leu |
| POAAN‐R5 | CCG (8) | Pro | T‐G/T‐G (8) | Trp/Leu | TGG (8) | Trp | TTG (8) | Leu | ||||
| Sensitive | CCG (8) | Pro | TGG (8) | Trp | ||||||||