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. 2002 Sep 2;21(17):4470–4479. doi: 10.1093/emboj/cdf471

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graphic file with name cdf471f1a.jpg — Fig. 1. Steady-state FRET between coumarin- and fluorescein-labeled SecA molecules. Coumarin- and fluorescein-labeled SecA heterodimers (dark gray traces) are compared with coumarin–SecA/unlabeled SecA (black traces) and fluorescein–SecA/unlabeled SecA (light gray traces) heterodimers. The following additions were made: (A) none; (B) 36 µg of unlabelled SecA; (C) 52 µg of BSA; (D) 24 µg of monomeric N68-SecA; (E) 5 µl of liposomes containing E.coli phospholipids; (F) 5 µl of DOPE/DOPG liposomes; (G) 5 µl of DOPE/DOPC liposomes; (H) 1.5 mg/ml ddm.

graphic file with name cdf471f1b.jpg — Fig. 1. Steady-state FRET between coumarin- and fluorescein-labeled SecA molecules. Coumarin- and fluorescein-labeled SecA heterodimers (dark gray traces) are compared with coumarin–SecA/unlabeled SecA (black traces) and fluorescein–SecA/unlabeled SecA (light gray traces) heterodimers. The following additions were made: (A) none; (B) 36 µg of unlabelled SecA; (C) 52 µg of BSA; (D) 24 µg of monomeric N68-SecA; (E) 5 µl of liposomes containing E.coli phospholipids; (F) 5 µl of DOPE/DOPG liposomes; (G) 5 µl of DOPE/DOPC liposomes; (H) 1.5 mg/ml ddm.