Fig. 5. Subcellular localization of the three different forms of miRNA. (A) RPA to detect miRNA. The indicated amounts of RNA from either HeLa or yeast cells were used for RPA. The asterisk indicates the self-protected probe. Schematically represented are the probes for the RPA and the protected fragments that are expected for each miRNA species. (B) Total, nuclear or cytoplasmic RNA from HeLa cells or yeast RNA was used for RPA.