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. 2005 Oct 21;1(4):e44. doi: 10.1371/journal.pgen.0010044

Figure 4. Iris Expression in D. melanogaster .

Figure 4

Iris expression through various stages of development was assayed using (A) RT-PCR and (B) Northern blots. Both show that Iris is predominantly expressed in adult females and males. (C) RT-PCR analysis on individually dissected tissues from adult flies shows that Iris is expressed in somatic tissues but expression is slightly reduced in ovaries and testes. RT-PCR to Karyopherin alpha-3 (αKap3, a ubiquitous nuclear import factor- CG9423) is shown as a control for amounts of template RNA in the RT-PCR reaction, and to show that there is no detectable contamination from genomic DNA.