Table 2.
16S rDNA sequence types obtained in this study.
Sequence type | No of clones in each librarya,b | Putative division | Database match (> 90% identity) | |
341/1492 | 515/1492 | |||
KB20 | 2 (2.4) | 0 (0) | Actinobacteria | No match |
KB11 | 2 (2.4) | 0 (0) | Low-G+C gram positive | 96% Syntrophomonas sp. MGB-C1 (AB021306) |
KB32 | 1 (1.2) | 0 (0) | Low-G+C gram positive | 91% Hydrocarbon-contaminated groundwater clone (AF050587) |
KU26 | 1 (1.2) | 24 (32.9) | Low-G+C gram positive | 90% Hydrocarbon-contaminated groundwater clone (AF050587) |
90% Desulfotomaculum thermobenzoicum DSM 6193 (AJ294430) | ||||
KB13 | 17 (21.0) | 2 (2.7) | Low-G+C gram positive | 94% Hydrocarbon-contaminated groundwater clone (AF050587) |
91% Toluene-degrading methanogenic consortium clone Eub 1 (AF423181) | ||||
91% Desulfotomaculum thermobenzoicum DSM 6193 (AJ294430) | ||||
KU25 | 0 (0) | 1 (1.4) | Low-G+C gram positive | 97% Acetobacterium paludosum DSM 8237 (X96958) |
KU21 | 0 (0) | 14 (19.2) | Low-G+C gram positive | 99% Groundwater clone Aspo4 (X95232) |
99% Acetobacterium malicum DSM 4132 (X96957) | ||||
KU8 | 0 (0) | 1 (1.4) | Low-G+C gram positive | No match |
KB35 | 1 (1.2) | 0 (0) | CFB group | 97% Marine clone agg32 (L10944) |
KU30 | 0 (0) | 1 (1.4) | CFB group | 90% Benzene mineralizing consortium clone SB-5 (AF029041) |
KB43 | 2 (2.4) | 0 (0) | Spirochete-likec | 94% Soil clone PBS-18 (AJ390485) |
KU13 | 0 (0) | 1 (1.4) | Spirochete-likec | No match |
KU34 | 1 (1.2) | 9 (12.3) | β-Proteobacteria | 96% Zoogloea ramigera ATCC 19544 (X74913) |
KB17 | 1 (1.2) | 0 (0) | β-Proteobacteria | 96% Hydrogenophaga palleronii DSM 63 (AF019073) |
KB38 | 1 (1.2) | 0 (0) | β-Proteobacteria | 97% Acidovorax defluvii BSB411 (Y18616) |
KB1 | 11 (13.6) | 1 (1.4) | δ-Proteobacteria | 98% Toluene-degrading methanogenic consortium clone Eub 5 (AF423185) |
96% Desulfovibrio aminophilus ALA-3 (AF067964) | ||||
KB33 | 1 (1.2) | 0 (0) | δ-Proteobacteria | 99% Desulfovibrio sp. Mlhm (AF193026) |
KB47 | 8 (9.9) | 0 (0) | δ-Proteobacteria | 91% Desulfovibrio sp. DR14 (Y17756) |
KU12 | 0 (0) | 2 (2.7) | δ-Proteobacteria | 97% Geobacter chapelleii 172 (U41561) |
KU32 | 0 (0) | 1 (1.4) | δ-Proteobacteria | 96% Chlorobenzene-contaminated groundwater clone GOUTB20 (AY050605) |
KB14 | 6 (7.4) | 0 (0) | δ-Proteobacteria | 94% Desulfobacula toluolica Tol2 (X70953) |
KB16 | 1 (1.2) | 0 (0) | ε-Proteobacteria | No match |
KB36 | 2 (2.4) | 0 (0) | ε-Proteobacteria | 99% Kuji groundwater clone 1070 (AB030590) |
KB8 | 1 (1.2) | 1 (1.4) | ε-Proteobacteria | 99% Kuji cluster-1 bacteria clone 1023 (AB030608) |
KB2 | 7 (8.6) | 2 (2.7) | ε-Proteobacteria | 99% Kuji cluster-1 bacteria clone 1033 (AB030608) |
KB21 | 1 (1.4) | 0 (0) | ε-Proteobacteria | 99% Kuji groundwater clone 1014 (AB030587) |
99% Geospirillium sp. KoFum (Y18254) | ||||
KB49 | 1 (1.4) | 0 (0) | ε-Proteobacteria | 99% Kuji groundwater clone 1065 (AB030598) |
94% Arcobacter cryaerophilus CCUG 17801 (L14624) | ||||
KB19 | 2 (2.4) | 0 (0) | Chlamydia group | No match |
KB4 | 1 (1.2) | 0 (0) | Nitrospira group | 94% Chlorobenzene-contaminated groundwater clone GOUTA19 (AY050588) |
KB40 | 2 (2.4) | 0 (0) | Green nonsulfur bacteria | 98% Hydrocarbon-contaminated groundwater clone WCHB1-44 (AF050565) |
KB44 | 1 (1.2) | 0 (0) | Green nonsulfur bacteria | 99% Trichlorobenzene-transforming consortium clone SJA-117 (AJ009488) |
97% Toluene-degrading methanogenic consortium bacterium Eub 4 (AF423184) | ||||
Archaea | 0 (0) | 5 (6.8) | ||
Chimera | 1 (1.2) | 0 (0) | ||
Total | 81 (100) | 73 (100) |