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. 1992 Dec;63(6):1455–1461. doi: 10.1016/S0006-3495(92)81733-2

Rod-like cholesterol micelles in aqueous solution studied using polarized and depolarized dynamic light scattering.

M A Castanho 1, W Brown 1, M J Prieto 1
PMCID: PMC1262259  PMID: 1489905

Abstract

Micelles of cholesterol in aqueous solution have been investigated using polarized and depolarized dynamic light scattering. They are shown to be highly extended and characterized by a narrow size distribution. It is shown that a rod-like model is applicable with length, L = 580 nm. Determination of the rotational diffusion coefficient by analysis of the autocorrelation function gave a value of theta = 150 s-1, which is close to the calculated value for the rod with this dimension. Depolarized dynamic light scattering measurements as a function of angle gave a value of 110 s-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Admirand W. H., Small D. M. The physicochemical basis of cholesterol gallstone formation in man. J Clin Invest. 1968 May;47(5):1043–1052. doi: 10.1172/JCI105794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolard J. How do the polyene macrolide antibiotics affect the cellular membrane properties? Biochim Biophys Acta. 1986 Dec 22;864(3-4):257–304. doi: 10.1016/0304-4157(86)90002-x. [DOI] [PubMed] [Google Scholar]
  3. Bruckdorfer K. R., Sherry M. K. The solubility of cholesterol and its exchange between membranes. Biochim Biophys Acta. 1984 Jan 11;769(1):187–196. doi: 10.1016/0005-2736(84)90022-1. [DOI] [PubMed] [Google Scholar]
  4. Castanho M. A., Coutinho A., Prieto M. J. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem. 1992 Jan 5;267(1):204–209. [PubMed] [Google Scholar]
  5. Craven B. M. Crystal structure of cholesterol monohydrate. Nature. 1976 Apr 22;260(5553):727–729. doi: 10.1038/260727a0. [DOI] [PubMed] [Google Scholar]
  6. Elias P. M., Friend D. S., Goerke J. Membrane sterol heterogeneity. Freeze-fracture detection with saponins and filipin. J Histochem Cytochem. 1979 Sep;27(9):1247–1260. doi: 10.1177/27.9.479568. [DOI] [PubMed] [Google Scholar]
  7. Gilbert D. B., Tanford C., Reynolds J. A. Cholesterol in aqueous solution: hydrophobicity and self-association. Biochemistry. 1975 Jan 28;14(2):444–448. doi: 10.1021/bi00673a035. [DOI] [PubMed] [Google Scholar]
  8. Haberland M. E., Reynolds J. A. Self-association of cholesterol in aqueous solution. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2313–2316. doi: 10.1073/pnas.70.8.2313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lonsdale K. Epitaxy as a growth factor in urinary calculi and gallstones. Nature. 1968 Jan 6;217(5123):56–58. doi: 10.1038/217056a0. [DOI] [PubMed] [Google Scholar]
  10. Loomis C. R., Shipley G. G., Small D. M. The phase behavior of hydrated cholesterol. J Lipid Res. 1979 May;20(4):525–535. [PubMed] [Google Scholar]
  11. Mazer N. A., Benedek G. B., Carey M. C. Quasielastic light-scattering studies of aqueous biliary lipid systems. Mixed micelle formation in bile salt-lecithin solutions. Biochemistry. 1980 Feb 19;19(4):601–615. doi: 10.1021/bi00545a001. [DOI] [PubMed] [Google Scholar]
  12. Newman J., Swinney H. L., Day L. A. Hydrodynamic properties and structure of fd virus. J Mol Biol. 1977 Nov 5;116(3):593–603. doi: 10.1016/0022-2836(77)90086-9. [DOI] [PubMed] [Google Scholar]
  13. Norman A. W., Demel R. A., de Kruyff B., van Deenen L. L. Studies on the biological properties of polyene antibiotics. Evidence for the direct interaction of filipin with cholesterol. J Biol Chem. 1972 Mar 25;247(6):1918–1929. [PubMed] [Google Scholar]
  14. Renshaw P. F., Janoff A. S., Miller K. W. On the nature of dilute aqueous cholesterol suspensions. J Lipid Res. 1983 Jan;24(1):47–51. [PubMed] [Google Scholar]
  15. Rogers J., Lee A. G., Wilton D. C. The organisation of cholesterol and ergosterol in lipid bilayers based on studies using non-perturbing fluorescent sterol probes. Biochim Biophys Acta. 1979 Mar 23;552(1):23–37. doi: 10.1016/0005-2736(79)90243-8. [DOI] [PubMed] [Google Scholar]
  16. Shieh H. S., Hoard L. G., Nordman C. E. Crystal structure of anhydrous cholesterol. Nature. 1977 May 19;267(5608):287–289. doi: 10.1038/267287a0. [DOI] [PubMed] [Google Scholar]
  17. Small D. M., Shipley G. G. Physical-chemical basis of lipid deposition in atherosclerosis. Science. 1974 Jul 19;185(4147):222–229. doi: 10.1126/science.185.4147.222. [DOI] [PubMed] [Google Scholar]
  18. Yeagle P. L., Bensen J., Greco M., Arena C. Cholesterol behavior in human serum lipoproteins. Biochemistry. 1982 Mar 16;21(6):1249–1254. doi: 10.1021/bi00535a022. [DOI] [PubMed] [Google Scholar]
  19. Yeagle P. L. Cholesterol and the cell membrane. Biochim Biophys Acta. 1985 Dec 9;822(3-4):267–287. doi: 10.1016/0304-4157(85)90011-5. [DOI] [PubMed] [Google Scholar]

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