Table 1.

PCR primer pairs used for RT-PCR confirmatory assays on S. mansoni gender-associated transcripts identified by cDNA microarray analysis

Accession number/gene name	PCR primer pairs	PCR product size
AI111005	5' - CTT CCG GAT GTA GAG GAT TTG- 3' 5' - CGG GAT ATG CGT TTG GAC TAG- 3'	301 bp
AI110935	5' - CGT TAA CAG TGC ACA GAT GCC- 3' 5' - GGA ATC CGA CTA CAT CAT TTC- 3'	182 bp
N21956	5' - CAG AAG AAC GAA ACT AAT CAC- 3' 5' - TTC TGA TGA TAC GAT ACC GTG- 3'	245 bp
N21941	5' - ATC ATC ATT ATG GTA CAT CAT- 3' 5' - TGA ATT TCA ACG TAT ATT CTC- 3'	236 bp
AI111017	5' - AAG TGT CCC TGA CAC AAC AAC- 3' 5' - GCC TGT TTC ATT TTC ACC TTC- 3'	137 bp
R95512	5' - TCG TTT ATT AGG AGG AAG ATC- 3' 5' - GCT TTC TGC AGC TTC GAG ACG- 3'	271 bp
AA559631	5' - TGT TAC ATA TAG TGG TGA GGG- 3' 5' - CGA CAC TTA CTG CCT GGA TGG- 3'	210 bp
AA559678	5' - GGT ATA TTT CGA GAA TGT GAG- 3' 5' - CGC TTA GGT TAA TCA TGA ATG- 3'	338 bp
Alpha tubulin	5' - GGC GGT GGT ACT GGT TCT GGG- 3' 5' - CAT TTA GCG CAC CAT CGA AGC- 3'	291 bp
Chorion	5' - GAA ACA GTC ACT CAC ACT CG- 3' 5' - ATG GCT GGG TTT GTA AGT GC- 3'	461 bp

RT-PCR reaction conditions are described in detail in the Materials and methods section. Thirty-five cycles were used for all primer pairs except for alpha-tubulin and clone AA559631 (23 cycles).