FIG. 3.

Efficiency of reverse transcription following inoculation of resting CD4⁺ T-cell subsets. Naive (□) and memory (•) CD4⁺ T cells were incubated at 37°C for 4 h with NL4-3 (dotted lines) or NL4-3/ICAM-1 (continuous lines), washed, and treated with 10 μM AZT for 16 h. Cells were washed twice to remove excess AZT, and cultures were maintained for 8 additional days. Cell-free supernatants were harvested at days 3, 6, and 9, and released viruses were quantified by p24 testing. Experiments were performed in triplicate, and standard deviations are indicated. Data shown are representative of six separate experiments made with different donors.