FIG. 6.

In vitro growth properties of PrV-ΔUL17F. (A) RK13 or RK13-UL17 cells were infected with PrV-Ka or PrV-ΔUL17F at an MOI of 10, harvested at the indicated times after infection, and titrated on RK13-UL17 cells. Average titers (PFU/ml) and standard deviations for three independent experiments are shown. (B) RK13 and RK13-UL17 cells were infected under plaque assay conditions with PrV-Ka and PrV-ΔUL17F and fixed 2 days postinfection. Infected cells were visualized by indirect immunofluorescence with a gC-specific monoclonal antibody.