FIG. 7.

Defect in cleavage of concatemeric viral DNA in the absence of PrV UL17. RK13 (A) or RK13-UL17 (B) cells were infected with PrV-Ka, PrV-ΔUL17F, or PrV-ΔUL28 at an MOI of 1. Cells were harvested at approximately 12 h p.i., and DNA was isolated, digested with BamHI, and separated on a 0.8% agarose gel. After transfer to nylon membranes, DNA was probed with labeled genome end-specific BamHI fragment 13. BamHI-13 hybridized to fragments BamHI-8′ and BamHI-13, which share homologous sequences since they are both derived from the inverted repeat regions (see Fig. 1), and the junction fragment composed of BamHI-13 and BamHI-14′, which is derived from head-to-tail concatemeric DNA.