Phylogenetic analysis of the relationship of KFV RdRp sequences to those of other virus families and viruses in the picornavirus superfamily. The phenogram is based on the alignment in Fig. 3C of polymerases from KFV and representative viruses of the Picornaviridae, Dicistroviridae, Comoviridae, Sequiviridae, and Caliciviridae families, as well as the unclassified insect virus APV. An unrooted neighbor-joining tree was inferred using the programs ClustalX 1.81 (58) and PHYLIP (19). All bifurcations with support in >700 out of 1,000 bootstraps are indicated. DCV, Drosophila C virus; RhPV, Rhopalosiphum padi virus; PSIV, Plautia stali intestine virus; PnPV, Perina nuda picorna-like virus; HAV, human hepatitis A virus; RTSV, rice tungro spherical virus; TobRV, tobacco ringspot virus.