FIG. 8.

Mapping determinants in V1 to V5 required for X4-mediated infection of macrophages. Infection of PBMC or macrophages from three independent donors and MT-2 cells mediated by six chimeric envelopes constructed with two, three, or four discontinuous hypervariable domains from subject 02 was compared to infection mediated by D-X4 V1 to V5 envelope from subject 02. Mean percent luciferase activity for each chimeric envelope relative to D-X4 parental envelope in each cell type was calculated and classified as in Fig. 7.