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. 2005 Nov;79(21):13298–13309. doi: 10.1128/JVI.79.21.13298-13309.2005

FIG. 2.

FIG. 2.

C189S can activate early but not late viral gene transcription. (A) Zta wt, C189S, or control expression vector was transfected into ZKO-293 cells and assayed by Western blot for expression of Zta, Rta, EA-D (BMRF1), or control PCNA. (B) RT-PCR analysis of mRNA for BcLF1 (capsid), BLLF1 (gp350), BRLF1 (Rta), BZLF1 (Zta), or cellular GAPDH control derived from untransfected ZKO-293 cells or transfected with control expression vector or expression vectors for Zta wt, C189S, or S186A at 24 h posttransfection. (C) RT-PCR analysis of mRNA as described for panel B with primers specific for BARF1, BSLF1, BBLF4, BHRF1, and GAPDH. (D) Luciferase assays were performed on 293 cells transfected with reporter plasmids for BRLF1p-Luc, BZLF1p-Luc, BHLF1p-Luc, or BMRF1p-Luc and with effector plasmids for Zta wt, C189S, or vector controls. M, molecular size marker.