FIG. 5.

Mapping of amino acids 118 to 141 of HAX-1 as a region of interaction with Vpr. (A) Confocal examination of expression and distribution of HAX-1 in HeLa cells. Fluorescent images indicate that the various HAX-1 deletion mutants are either capable or not capable of sequestering Vpr. (B) Diagrammatic summary of HAX-1 deletion mutant correlating the ability to sequester Vpr with ability to halt Vpr-induced apoptosis. Bcl2 homology domains 1 and 2 (BH1 and -2, green), PEST sequence (PEST, red), and transmembrane domain (TMD, brown) are indicated.