TABLE 2.

Neutralizing antibody activity against a panel of six subtype B and seven subtype C HIV strains

Species	Immunization group	Animal no.	% Reduction in RLUa
			6101.10 B	QH0692.42 B	PVO.4 B	3988.25 B	6535.3 B	BG1168.1 B	Du123.6 C	Du151.2 C	Du156.12 C	Du172.17 C	Du422.1 C	ZM18108M.6 C	ZM14654M.7 C
Macaque	gp140ΔV2 TV1 + Gag	1.1	0	2	0	0	44	0	0	0	0	6	0	30	0
		1.2	0	5	0	0	32	1	0	1	8	8	0	10	11
		1.3	32	55	45	52	68	34	67	64	64	54	35	53	46
		1.4	5	0	0	20	10	1	13	0	0	41	11	18	5
	Gag + Pol	2.1	0	12	0	22	35	0	15	0	27	27	18	30	26
		2.2	30	31	62	26	44	13	8	17	38	10	9	25	21
		2.3	0	0	0	0	0	0	0	0	0	0	0	12	0
	Gag − Pol	3.1	15	0	13	31	0	10	18	0	29	12	11	13	5
		3.2	0	0	0	35	35	0	7	0	47	1	21	28	23
		3.3	11	46	7	9	26	0	24	14	41	20	9	31	24
		3.4	0	23	0	26	42	0	26	8	24	23	13	23	23
Rabbit	gp140ΔV2 SF162 (group 1)	1.1	0	0	0	10	65	0	6	0	20	10	1	20	38
		1.2	27	54	36	41	60	31	24	15	36	15	35	24	43
		1.3	0	0	0	0	49	0	0	0	0	0	0	0	0
		1.4	56	62	51	0	66	30	0	1	0	0	39	0	41
	gp140 TV1 (group 5)	5.1	0	0	0	0	0	0	0	0	0	0	0	0	0
		5.2	0	0	0	0	2	0	0	0	10	0	1	0	2
		5.3	0	13	0	0	53	0	0	0	0	0	0	0	16
		5.4	5	0	0	13	54	0	5	0	0	0	0	0	46
	gp140ΔV2 TV1 (group 6)	6.1	0	18	0	16	28	0	5	0	0	0	0	0	13
		6.2	0	0	0	0	15	0	0	0	0	0	0	0	0
		6.3	22	24	0	24	46	6	17	12	45	27	33	14	50
	gp140ΔV2 TV1+SF162 (group 8)	8.1	0	0	0	3	42	0	22	12	16	16	8	32	38
		8.2	18	26	0	0	50	6	51	5	53	24	3	27	25
		8.3	0	17	0	0	57	0	0	0	0	0	0	13	19
		8.4	0	39	0	0	71	0	46	8	6	32	7	39	40

^aPostprotein samples (i.e., 2 weeks after the fifth immunization) were screened at a 1:15 dilution in triplicate in TZM-bl cells. Values are the percent reduction in RLU relative to the corresponding preimmune sera (in rabbits) or post-DNA sera (in macaques). Neutralizing activity values of ≥50% reduction in RLU are indicated in boldface.