TABLE 1.
Number of positive-strand RNA, negative-strand RNA, core, NS4B, and NS5B molecules per Huh-7 cell
| HCV sequence | Mean no. of molecules/cell ± SDa
|
||||
|---|---|---|---|---|---|
| Negative-strand | Positive-strand | Core | NS4B | NS5B | |
| Con1/ET (transient transfection)b | 40 ± 4 | (2.0 ± 0.3) × 102 | (4.9 ± 1.4) × 106 | (7.7 ± 1.8) × 105 | (1.5 ± 0.4) × 106 |
| Monocistronic replicon (cell clone 11-1) | 81 ± 11 | (4.4 ± 1.5) × 102 | NA | (8.9 ± 3.2) × 105 | (2.2 ± 0.8) × 106 |
| Bicistronic replicon (cell clone 9-13) | 94 ± 31 | (7.6 ± 2.9) × 102 | NA | (8.0 ± 0.4) × 105 | (1.2 ± 0.5) × 106 |
a
Data represent mean values and standard deviations of samples harvested at 48, 72, and 96 h after seeding. NA, not applicable.
b
Per-cell data was not normalized for transfection efficiency, which was routinely 50 to 80%.