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. 2005 Nov;79(21):13326–13337. doi: 10.1128/JVI.79.21.13326-13337.2005

FIG. 7.

FIG. 7.

Impact of Simvastatin on whole-cell concentration and raft distribution of LMP1, galectin 9, and TRAF3 in NPC cells. C15 cells derived from xenografted tumors were short-term cultured for 5 days on a polyHEMA matrix in the presence of 5 μM Simvastatin or DMSO alone and then subjected to protein extraction and a raft flotation assay. (a) Raft flotation assay of C15 cells treated by Simvastatin (Sim) or the vehicle (DMSO) by itself. The flotation assay generates three cell fractions in addition to the raft fraction: LSP and HSP containing mostly cytoskeletal elements and a fraction called F40 recovered in the 40% sucrose layer of the step gradient. F40 contains most components of the cytosol and nonraft membranes (11). Ten micrograms of proteins was loaded per lane. Parallel blots were done for detection of LMP1, TRAF3, and galectin 9. For all three proteins, the ratio of protein concentration in the rafts compared to the initial extract is not significantly different between Simvastatin-treated and control cells. (b) Comparison of whole-cell concentrations of LMP1, galectin 9, and TRAF3 in C15 cells treated for 5 days by Simvastatin (Sim) or the vehicle (DMSO) by itself (30 μg of protein per lane). An approximate twofold increase is observed in the whole-cell concentrations of LMP1 and galectin 9 in C15 cells treated by Simvastatin compared to the control.