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Short-time replication assay (see Materials and Methods). (A) Deletion of amino acids 1139 to 1143 resulted in LANA-1 constructs unable to replicate a TR-containing plasmid. (B) The C terminus of LANA-1 was able to replicate a TR-containing plasmid, although at a reduced level compared to that of full-length LANA-1. (C) Deletion of LANA-1 internal repeat and flanking regions did not affect LANA-1-mediated replication of a TR-containing plasmid.
