FIG. 7.

Dimerization of LANA-1 deletion mutants. GST and GST-CT fusion protein were expressed in bacteria as explained in Materials and Methods. LANA-1 proteins were in vitro transcribed/translated, incubated with GST or GST-CT, and bound to Sepharose beads for 1 h. Following extensive washing steps, the beads were boiled in loading buffer containing β-mercaptoethanol, and the samples were separated by SDS-PAGE (see Materials and Methods). The detection of LANA-1 proteins and GST-CT was carried out with a KSHV-positive patient serum.