TABLE 7.

Only acidic amino acids can rescue assembly of hybrid core particles containing a positively charged insert^a

OMP-2 epitopeb	Sequenceb	Linker type	Insert pI	Assembly resultc
L	L-EDANGTRDHKKGRHT-L	Small, hydrophobic, nonpolard	8.74	−
EE	EE-EDANGTRDHKKGRHT-EE	Acidic	4.88	+
DD	DD-EDANGTRDHKKGRHT-DD	Acidic	4.67	+
P	P-EDANGTRDHKKGRHT-P	Bulky, hydrophobic, nonpolar	8.74	−
QQ	QQ-EDANGTRDHKKGRHT-QQ	Amide, hydrophobic, uncharged, polar	8.74	−
TT	TT-EDANGTRDHKKGRHT-TT	Nucleophilic, uncharged, polar	8.74	−
YY	YY-EDANGTRDHKKGRHT-YY	Aromatic, hydrophobic, uncharged, polar	8.56	−

^aThe C terminus used for OMP-2 hybrid core particles was HyW2.

^bE, glutamic acid; D, aspartic acid; P, proline; Q, glutamine; T, threonine; Y, tyrosine.

^cFor explanation of assembly scores (+, −), see Materials and Methods.

^dLeucine linker due to cloning restriction site.