FIG. 2.

Recombinant VSV with mutations in the L gene. The plaque morphology of each of the recombinant viruses is shown compared to rVSV. Note that plaques of K1651A, D1762A, K1795A, E1833A, E1833Q*, and E1833Q were developed after 48 h of incubation compared to rVSV and G1674A, which were developed after 24 h. Differing dilutions of the small plaque viruses were plated to emphasize the plaque morphology. The sequence of the modified region for each mutant virus is shown. Note that the sequence trace shown is negative sense for K1651A, G1674A, D1762A, and K1795A and positive sense for E1833A and E1833Q.