FIG. 8.
Effect of L gene mutations on viral protein synthesis in BHK-21 cells. (A) BHK-21 cells were infected with the wild-type and mutant viruses at an MOI of 3. Proteins were labeled by incorporation of [35S]methionine-cysteine in the presence of actinomycin D as described in Materials and Methods. Cytoplasmic extracts were prepared, and proteins were analyzed by SDS-PAGE and detected by using a phosphorimager. Extract from equivalent numbers of cells was loaded in each lane. For K1795A, viral proteins were labeled either at 3, 6, 9, or 12 h postinfection. The infecting virus is indicated above the lanes, along with the time postinfection at which the labeling commenced. The identity of the proteins is shown on the left. (B) Three independent experiments were used to generate the quantitative analysis shown. For each protein the mean ± the standard deviation was expressed as a percentage of that observed for rVSV.