TABLE 2.

Summary of phenotypic properties of VSV L gene mutants

Mutant	Mean plaque size (mm) ± SDa	Titer at 24 h (log10 PFU/ml)	RNA synthesis (% rVSV)		Protein (% rVSV)c	% 7mG	% Am
			Cells	In vitro
rVSV	4.0 ± 0.5*	9.7 ± 0.3	100	100	100	97	100
K1651A	0.9 ± 0.2†	6.2 ± 0.2	60-120b	75	100	<1	<1
G1674A	4.1 ± 0.8*	9.6 ± 0.6	>100	100	100	96	96
D1762A	1.1 ± 0.2†	8.1 ± 0.1	50	75	40-100*	<1	<1
K1795A	0.7 ± 0.2†	6.3 ± 0.2	10	40	10†	<1	<1
E1833A	0.8 ± 0.2†	6.0 ± 0.1	50	60	40-100*	11	5
E1833Q*	1.2 ± 0.2†	7.8 ± 0.2	50	100	40-100*	<1	<1
E1833Q	1.3 ± 0.2†	7.9 ± 0.2	NDd	100	ND	<1	<1

^a*,Plaque diameter was measured at 24 h postinoculation; †,plaque diameter was measured at 48 h postinoculation.

^bThe percentage of each mRNA varied: N, 80%; P/M, 60%; G, 80%; and L, 120%.

^c*, N, P, and M proteins were 100% of rVSV levels (G, 70%; L, 40%). †, At later times postinfection, the levels of L and G protein were specifically reduced.

^dND, not determined.