FIG. 5.

Inhibition of HCV RNA replication by antiviral compounds. (A and B) Effect of a nucleosidic, NS5B-specific inhibitor on the maintenance of HCV RNAs in HuH6 Con1 cells and Huh-7 cells of clone 9-13, respectively. Cells were seeded into multiple cell culture dishes; on the following day, the culture medium was supplemented with 1 μM 2′-C-methyladenosine. At given time points, cells were harvested, total RNA was prepared, and the number of HCV RNA copies was determined by quantitative, real-time RT-PCR. The result of a single representative experiment is shown. (C and D) Antiviral effect of a nonnucleosidic, NS5B-specific inhibitor of HCV replication. The experiment was performed and analyzed as described above.