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Effect of IFN-γ on STAT1 expression and localization in HuH6 and Huh-7 cells. Naive HuH6 (A) and Huh-7 (B) cells were seeded onto glass coverslips, cultivated for 16 h in normal culture medium, and further incubated for 24 h in the absence of cytokines (left) or in the presence of 1,000-IU/ml IFN-γ (right). After fixation and permeabilization, cells were immunostained for STAT1. Bars, 25 μm.
