Table 2.

Agrin and LN1 aggregation stimulus protocols. Note that the medium for all cultures was changed at t = 24 hrs, either to fresh medium or medium with stimulus.

Treatment	Abbreviation	Stimulus
1) Control	C	None
2) Agrin alone	A	Agrin 24–48 hrs
3) LN1 alone	L	LN1 24–48 hrs
4) Agrin + LN1	AL	Agrin+LN1 24–48 hrs
5) Agrin then LN1	A>L	Agrin 24–26 hrs: LN1 26–48 hrs
6) LN1 then Agrin	L>A	LN1, 24–26 hrs: Agrin 26–48 hrs