FIG. 2.

Effect of S-phase on DEN2 titers. Asynchronously cycling C6/36 cells or Huh-7 cells were mock infected or infected with DEN2 strains N1042, C0477, or 16681 for 2 h, washed, and then cultured in growth medium containing either PBS or 2 mM THY for an additional 38 h (C6/36) or 28 h (Huh-7). (A and C) Cell cycle profiles. Cell cycle analysis was carried out as described for Fig. 1. Representative profiles are shown. Percentages of cells in G₁-, S-, and G₂/M-phases were averaged from at least three experiments ± the standard deviation (SD). (B and D) Virus titers. Titers were measured by plaque assay. Cell number was accounted for when calculating relative virus output for PBS- and THY-treated populations. An average of at least three experiments, each done in duplicate, ± the SD is shown. NS, not significantly different than 1.