Figure 2.

Generation and analysis of modified RevTetOFF constructs. (A) Three additional constructs were generated in which the CMV promoter was replaced by either the elongation factor 1-alpha or phosphoglycerate kinase-1 promoters, or a tetracycline responsive element. (B) Parental HC11 cells were transiently co-transfected with the indicated constructs and maintained for 48 hours in the presence of absence of 1 μg/ml doxycycline. The cells were fixed and stained for β-galactosidase expression using X-gal 48 hours after transfection.