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Northern blot analysis of mRNA from β-galactosidase SXA reporter constructs. Steady-state β-galactosidase mRNA levels were measured in the wild-type, [PSI+], upf1Δ, and [PSI+]/upf1Δ strains. The β-galactosidase SXA reporter constructs contained either a UAA, UAG, or UGA stop codon, or a UGG tryptophan codon in the readthrough position.
