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Corrected readthrough levels determined using the β-galactosidase readthrough assay. The fold increase in readthrough in the [PSI+], upf1Δ, and [PSI+]/upf1Δ strains are represented relative to the basal level of readthrough measured in the wild-type strain at the corresponding stop codon. (A) SXA β-galactosidase reporter constructs. (B) QXQ β-galactosidase reporter constructs. WT, wild type.
