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Context-dependent readthrough (QXQ/SXA) determined using the β-galactosidase readthrough assay. The data is expressed as the ratio of readthrough measured with the β-galactosidase QXQ reporter relative to the readthrough measured with the β-galactosidase SXA reporter. The QXQ/SXA ratios are shown for the wild-type, [PSI+], upf1Δ, and [PSI+]/upf1Δ yeast strains. WT, wild type.
