FIGURE 7.

Correlation between mRNA levels and corrected readthrough of stop codons using the β-galactosidase readthrough assay. The corrected readthrough values obtained in both the β-galactosidase SXA and QXQ contexts were plotted against the relative level of LacZ mRNA for each construct as determined by northern blot analysis. (A) Plot of data obtained with the wild-type strain, SXA context (squares); wild-type strain, QXQ context (diamonds); [PSI⁺] strain, SXA context (circles); and [PSI⁺] strain, QXQ context (triangles). (B) Plot of data obtained with the upf1Δ strain, SXA context (squares); and the upf1Δ strain, QXQ context (triangles). WT, wild type.