TABLE 1.
Effect of stop codon context on [PSI+] and upf1Δ-mediated readthrough
| Strain | Reporter construct | Readthrough codon | Absolute β-gal activity (±SD)a | Relative β-gal mRNA (±SD)b | Corrected readthrough (%) |
| Wild type | SXA | UGG | 193.33 (±15.40) | 1 | 100 |
| Wild type | SXA | UAG | 0.023 (±0.067) | 0.66 (±0.036) | 0.018 (±0.003) |
| Wild type | SXA | UGA | 0.019 (±0.004) | 0.71 (±0.088) | 0.015 (±0.001) |
| Wild type | SXA | UAA | 0.016 (±0.007) | 0.53 (±0.025) | 0.016 (±0.004) |
| [PSI+] | SXA | UGG | 188.30 (±12.23) | 1 | 100 |
| [PSI+] | SXA | UAG | 0.088 (±0.029) | 0.44 (±0.058) | 0.11 (±0.011) |
| [PSI+] | SXA | UGA | 0.030 (±0.009) | 0.50 (±0.078) | 0.039 (±0.010) |
| [PSI+] | SXA | UAA | 0.024 (±0.010) | 0.50 (±0.052) | 0.025 (±0.006) |
| upf1Δ | SXA | UGG | 232.65 (±15.36) | 1 | 100 |
| upf1Δ | SXA | UAG | 0.184 (±0.034) | 0.85 (±0.11) | 0.056 (±0.008) |
| upf1Δ | SXA | UGA | 0.094 (±0.001) | 1.06 (±0.093) | 0.038 (±0.007) |
| upf1Δ | SXA | UAA | 0.171 (±0.029) | 1.12 (±0.11) | 0.063 (±0.005) |
| [PSI+]/upf1Δ | SXA | UGG | 163.71 (±36.33) | 1 | 100 |
| [PSI+]/upf1Δ | SXA | UAG | 0.249 (±0.025) | 1.06 (±0.062) | 0.15 (±0.022) |
| [PSI+]/upf1Δ | SXA | UGA | 0.177 (±0.027) | 1.18 (±0.11) | 0.096 (±0.009) |
| [PSI+]/upf1Δ | SXA | UAA | 0.323 (±0.037) | 1.17 (±0.041) | 0.18 (±0.025) |
| Wild type | QXQ | UGG | 549.74 (±54.17) | 1 | 100 |
| Wild type | QXQ | UAG | 3.59 (±0.18) | 0.77 (±0.050) | 0.83 (±0.054) |
| Wild type | QXQ | UGA | 3.68 (±0.21) | 0.71 (±0.11) | 1.04 (±0.15) |
| Wild type | QXQ | UAA | 1.85 (±0.15) | 0.62 (±0.083) | 0.57 (±0.085) |
| [PSI+] | QXQ | UGG | 484.91 (±41.67) | 1 | 100 |
| [PSI+] | QXQ | UAG | 16.82 (±1.21) | 0.87 (±0.12) | 4.43 (±0.47) |
| [PSI+] | QXQ | UGA | 14.63 (±1.52) | 0.76 (±0.11) | 3.97 (±0.41) |
| [PSI+] | QXQ | UAA | 9.18 (±0.64) | 0.83 (±0.11) | 2.6 (±0.22) |
| upf1Δ | QXQ | UGG | 559.10 (±34.39) | 1 | 100 |
| upf1Δ | QXQ | UAG | 10.96 (±1.13) | 1.29 (±0.18) | 1.64 (±0.25) |
| upf1Δ | QXQ | UGA | 12.70 (±1.27) | 1.16 (±0.23) | 2.33 (±0.23) |
| upf1Δ | QXQ | UAA | 9.23 (±1.23) | 0.98 (±0.058) | 1.81 (±0.14) |
| [PSI+]/upf1Δ | QXQ | UGG | 204.88 (±45.94) | 1 | 100 |
| [PSI+]/upf1Δ | QXQ | UAG | 24.60 (±2.86) | 2.02 (±0.089) | 5.75 (±0.70) |
| [PSI+]/upf1Δ | QXQ | UGA | 25.90 (±4.22) | 1.97 (±0.18) | 6.87 (±0.39) |
| [PSI+]/upf1Δ | QXQ | UAA | 7.90 (±2.12) | 1.86 (±0.057) | 2.17 (±0.28) |
aEight independent β-galactosidase assays were carried out for each construct.
bAt least three independent mRNA measurements were carried out for each construct.