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. Author manuscript; available in PMC: 2005 Oct 21.
Published in final edited form as: J Biol Chem. 2001 Feb 7;276(18):14855–14860. doi: 10.1074/jbc.M010097200

Fig. 4. The effect of the sodium binding site on the endothelin-1 inhibition of Kir3 homomers.

Fig. 4

Oocytes were injected with a mixture of the following cRNAs: MOR (1 ng), HETAR (1 ng), and the Kir form listed below the bar (1 ng each). Recordings were performed 2–3 days post-injection. The experiment was performed as in Fig. 2. The bar graph compares Kir3.1(F137S) versus Kir3.1(F137S)(N217D) with addition of a site for Na+-dependent gating. The second set of bars are Kir3.4(S143T) compared with Kir3.4(143T)(D223N); a mutant with a deletion of the Na+ activation site. Data are means ± S.E. from four to seven oocytes and two to three independent experiments (**, p < 0.01).