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. 2025 Nov 18;14:RP107718. doi: 10.7554/eLife.107718

Figure 5. pou4-2 expression is required for mechanosensory neuron regeneration and function.

Figure 5.

(A) Acetylated-tubulin staining shows decreased cilia signal in the dorsal ciliated stripe of pou4-2(RNAi) animals. Scale bars = 200 μm, n = 4 worms stained for each of the control and experimental groups. (B) Higher magnification confirms stripe reduction; epidermal and ventral cilia are unaffected. Scale bars = 25 μm. (C) Vibration response assay demonstrating body contractions in wild-type animals following tapping stimulus. (D, E) Quantification of vibration response assay data shows significantly reduced contraction responses in both intact (D) and regenerate (E) pou4-2(RNAi) animals. Data in D and E are represented as mean ± SD; n ≥25 worms for each experimental group. ****p<0.0001, Student’s t-test. (F) Model: pou4-2 acts downstream of soxB1-2 in regulating mechanosensory neuron differentiation.