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. Author manuscript; available in PMC: 2005 Oct 21.
Published in final edited form as: J Biol Chem. 2003 Apr 7;278(24):22119–22127. doi: 10.1074/jbc.M212822200

Fig. 8. Requirement of pRb for efficient ER coactivation function of PELP1.

Fig. 8

A, pRb-defective SAOS2 cells were cultured for 48 h in 5% DCC serum and then transfected with the ERE reporter, ERα, and PELP1 with or without pRb expression vectors. Cells were treated with or without E2 (10−9 m) after 48 h, and luciferase (luc) activity was measured after 24 h of ligand treatment. The inset shows the expression of endogenous PELP1 in SAOS2 cells. B, SAOS2 cells were cotransfected with the PELP1 N-terminal fragment and the pRb expression vector, and ERE-luciferase activity was measured after 24 h of E2 treatment. Con, control; WT, wild-type; PELP-MT, PELP-1 protein containing amino acids 1–605.