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. Author manuscript; available in PMC: 2005 Oct 21.
Published in final edited form as: J Clin Endocrinol Metab. 2004 Dec;89(12):6130–6138. doi: 10.1210/jc.2004-0909

Fig. 3.

Fig. 3

PELP1 down-regulation by siRNA reduces E2-mediated responses in Ishikawa cells. A, Ishikawa cells were treated with nonspecific or PELP1-specific siRNAs. Total cell lysates were analyzed by Western blotting using the PELP1 antibody. B, Ishikawa cells were transfected with ERE reporter gene along with nonspecific siRNA or PELP1-specific siRNAs. After 48 h, cells were treated with or without E2 (10−9 m) for 16 h, and the reporter gene activity was measured. C, Ishikawa cells were transfected with nonspecific or PELP1-specific siRNAs, treated with or without E2 (10−9 m), and cell number was counted after 72 h. D and E, PELP1 promotes tamoxifen (Tam)-mediated agonist action in the endometrium. Ishikawa cells (D) and MCF-7 cells (E) were transfected with 3XERE reporter gene along with or without PELP1 expression vector (Vec). After 48 h, cells were treated with either E2 (10−9 m) or Tam (10−8 m), and the reporter gene activity was measured after 24 h. F, Ishikawa cells were treated with or without Tam, lysates were immunoprecipitated with ERα antibody, and the presence of PELP1 in the immunoprecipitates was analyzed by Western blotting.