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. Author manuscript; available in PMC: 2006 Jul 1.
Published in final edited form as: Cancer Res. 2005 Jul 1;65(13):5571–5577. doi: 10.1158/0008-5472.CAN-04-4664

Figure 3.

Figure 3

PELP1 selectively modulates STAT3 phosphorylation at Ser727. COS-1 cells (A) and murine fibroblast cells (B) were cotransfected with STAT3 with or without PELP1. After 24 hours, the cells were serum starved for an additional 24 hours and then treated with EGF for 30 minutes. The status of STAT3 phosphorylation was analyzed with phosphospecific antibodies. C, MCF-7 cells were transfected with the control or PELP1-specific siRNA and then treated with EGF for 30 minutes. Total cell lysates were subjected to Western blotting with phospho-STAT3 Ser727–specific antibody. D, MCF-7 cells were transfected with control or PELP1-specific siRNA. After 48 hours, the cells were serum starved for 24 hours and treated with EGF (100 ng/mL) for 2 hours. Total RNA was isolated and expression of cyclin D1 was analyzed by Northern blot analysis. E, HeLa cells were transfected with control or PELP1-specific siRNA. After 48 hours, the cells were serum starved for 24 hours and treated with EGF (100 ng/mL) for 2 hours. Total RNA was isolated and expression of c-fos was analyzed by Northern blot analysis.